Research Progress in Area 2 方向 ( 二 ) 課題進展 113 Abstract The microbiome characterization may be used as an important bio-indicator to assess the host health. This study will be an initial description and discussion of the kidney, gills and liver microbial communities of five common fish species sampled from multiple site along the sea basins of Hong Kong and Israel. The goals will be to establish a baseline dataset of the microbial communities associated with the tissues of wild common marine fish in South China Sea and Eastern Mediterranean Sea; to identify fish species-specific microbiome characteristics; to examine fish organ microbiome in search of candidate pathogens; and to develop sample and analytical method that will be used for long-term, consistent monitoring in our study areas and which will be incorporated in other marine fish pathogens surveys in other regions in the world. Research Activities and Progress Due to Covid-19 outbreak, Dr. Morick could not recruit students last year, therefore some of the planned experiments will be done this year. • Extracted DNA via several different methods; • Constructed specific molecular systems for a few bacterial pathogens; • Constructed a new generation sequencing (NGS) molecular system; • Finalized the work and published papers. Key Findings • Different pathogenic bacteria were detected in a few marine fish species; • The overall prevalence for Vibrio species in wild fish in 2018 was higher than in 2019; • No significant difference was detected for Streptococcus species’ prevalence in wild fish between 2018 and 2019; • Photobacterium damselae was detected in four marine fish species; • More pathogenic bacteria in gill samples were detected than in kidney and liver samples. Research Output Publication 1 Trained personnel 4 Specific Pathogens and Microbial Abundance within Liver, Gills and Kidney Tissues of wild Marine Fish in South China Sea and Eastern Mediterranean Sea Dr. Danny Morick University of Haifa Fig 1. Relative abundance of major bacterial pathogens families detected in gills of wild marine fish collected alone the eastern Mediterranean Sea, Israel.
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