232 4. Pearl River Estuary - South China Sea Cruise Trip 珠江口-南海航次 Abstract: With the full support of the Hong Kong Branch (HKB) of the Southern Marine Science and Engineering Guangdong Laboratory (Guangzhou), 24 scientists from the area 1 of the HKB and the South China Sea Institute of Oceanology have accomplished a cruise to the Pearl River Estuary and the South China Sea during 28 June – 10 July, 2021. In this cruise, we aimed to understand the impacts of anthropogenic input and warming to marine life processes, with specific focus on the planktonic food web, microbial loop and microbe-driven biogeochemical processes. Therefore, we have conducted a series of observations and incubation experiments along a transect from the highly urbanized and eutrophic Pearl River Estuary to the offshore water in the shelf of the South China Sea, which is expected to display an obvious gradient of the anthropogenic inputs (e.g., nitrogen loading) from the river discharge (Figure 1). In total, we have 9 stations along the transect, in which time series observations were conducted at 3 stations that represented distinct water bodies (P01: estuarine water; EP03: transitional zone; A02: offshore oceanic water). At these 3 stations, samples for diverse biogeochemical parameters were collected every 3 hours for 24 hours (9 time-points in total). Besides, we have conducted temperature manipulating incubation experiments at all stations except station A04, in which the responses of different life processes were studied under different temperatures (-5°C, -3°C, ambient, +3°C, +5°C). 简介：香港分部方向(一)团队执行为期12 天的珠江口及邻近海域航次。本航次聚焦 生态系统对环境变化的响应，通过海上观 察与实地实验，研究初级生产者与次级生 产者及微生物之间的相互作用，水层与沉 积物元素循环之间的关系等等关键科学问 题。科考人员在该断面总共做了9个站位， 其中包含3个代表不同水体的时间连续站。 在这3个连续站，每隔3小时采集生地化参 数，持续24小时(共计9次采样)。此外，在 所有站位(除A04)进行了控温培养实验，研 究不同生物过程在不同温度下的响应。 Date 日期 : 2021. 06. 28 – 07. 10
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