School of Science Division of Life Science 18 Chinese Herbal Medicine for General Health Supervisor: LIANG Chun / LIFS Student: KWON Daye / BIOT PARK Joo Hyoung / BIOT Course: UROP2100, Fall UROP1100, Fall Traditional Chinese Medicine has been studied to have various applications in diseases and disorders, including that of the metabolic syndrome. In the present experiment, the efficiencies in the treatment of insulin resistance of three popular TCMs—Astragalus membranaceus, Poria cocos, Taraxacum were compared to that of a commonly known diabetes drug, Metformin. Insulin-resistant HepG2 cells were treated with each drug solution and were screened with addition of a glucose assay kit. Absorbance at 490 nm of the treated HepG2 was measured and a western blotting analysis was performed for quantification of Akt protein. Although Taraxacum showed the highest uptake of glucose, the results also suggested high efficiencies of glucose uptake stimulation and potentials of Astragalus membranaceus and Poria cocos. Chinese Herbal Medicine for General Health Supervisor: LIANG Chun / LIFS Student: NG Cheuk Yan Gloria / SSCI Course: UROP1000, Summer Diabetes is a major global medical issue that jeopardizes general public health. There is an increasing interest on the approach of Traditional Chinese Medicines (TCM) to complement diabetes treatment in Western medicines. The study aims to evaluate the effect of some TCM, Momordica Charantia (MC), Glycyrrhiza Uralensis (GU), and Ginseng (GS), on insulin resistance (IR) of HepG2 cells. The glucose uptake of drug-treated cells is measured to test the anti-insulin-resistant effect of drugs. The experiment results show that MC, GU, and GS exhibit an anti-insulin-resistant impact, where its effectiveness increases with increasing concentration and during drug treatment. In conclusion, using TCM as an anti-diabetic treatment can be a possible approach in Western therapies for diabetes. Chinese Herbal Medicine for General Health Supervisor: LIANG Chun / LIFS Student: TSANG Chun Kit / SSCI Course: UROP1000, Summer Certain herbal medicines have been used in Traditional Chinese medical practices to treat diabetic symptoms. Astragalus membranacues and Codonopsis pilosula are considered to possess hypoglycemic effects in a dose dependent manner 48 hours after adding to insulin resistance HepG2 cells. This effect does not manifest a dose dependent manner in the 24 hours trial which might be related to experimental flaws. The usage of TCMs demonstrates much smaller toxicity to HepG2 cells compared to typical anti-diabetic medicine metformin.
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