School of Science Department of Chemistry 3 Construction and Application of Surface Enhanced Raman Spectrometer in Biomolecules Characterization Supervisor: HUANG, Jinqing / CHEM Student: LI, Hiu Fung / SSCI Course: UROP1000, Summer Cancer cells were being optically trapped by Optical Tweezer and scanned by Raman spectroscopy. The Raman signal of the cancer cells were found to be the strongest at the centre of it by measuring its Raman signal along a horizontal axis. Raman signals were collected from the centre of the cancer cells which several spectrum peaks corresponded to the macromolecules of the cancer cells were located. The data collected can be further used to observe the spectra change of the cancer cells when they are being treated with anticancer drugs. The mechanism and effectiveness of the anti-cancer drugs can then be revealed, improving the development of anti-cancer drugs. Construction and Application of Surface Enhanced Raman Spectrometer in Biomolecules Characterization Supervisor: HUANG, Jinqing / CHEM Student: PARK, Sejeong / CHEM-IRE Course: UROP1100, Fall This research studied the biomolecular composition of breast ductal carcinoma tissue using Raman imaging, with the hope of contributing to the development of a breast cancer diagnostic tool based on Raman spectroscopy. Raman images were generated using unsupervised machine learning, which assisted in the clustering of spectra. Peak ratio analysis of the mean spectra of each cluster obtained in the high wavenumber region showed transitional trends in proteins and lipid components, allowing several interpretations of our ductal carcinoma tissue. The lipid ratio gradient from our study suggests the possibility of enhanced accumulation of certain fatty acids in cancer-associated fibroblasts (CAFs) surrounding breast cancer cells. This study has recently proceeded to use Raman imaging to monitor the binding interactions between cancer cells and T-cells. Medicinal Chemistry on Novel Type I1/2 ALK Inhibitors for Combating Drug-resistant Mutants Supervisor: HUANG, Yong / CHEM Student: YUNG, Zhe Jamie / CHEM-IRE Course: UROP1100, Summer Bifunctional N-heterocyclic carbenes (NHC) are an emerging class of NHC catalysts, where the underlying HOMO-raising activation of the nucleophile from the carbene works in conjunction with the LUMO-lowering activation of the electrophile from the hydrogen-bonding donor. This system has shown greater efficiency than the established tertiary amine-thiourea scaffold system, due to the higher basicity of the carbene. This type of bifunctional catalyst has proven effective in catalyzing asymmetric hetero-Michael addition reactions, such as aza-Michael addition. Novel designs of such catalysts may be able to catalyze asymmetric intermolecular oxa-Michael addition of alcohols, as well as asymmetric 1,6-conjugate addition through the extended conjugated system.
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