School of Science Division of Life Science 14 Molecular Regulation of Skeletal Muscle Stem Cell Quiescence and Activation Supervisor: CHEUNG Tom / LIFS Student: YOON Dayeong / BCB Course: UROP 1100, Fall The objective of this study is to examine the effects of asymmetric cell division on self-renewal, differentiation, and activation processes in satellite cells, a population of skeletal muscle stem cells. The idea of the immortal strand, which could be crucial in preserving stemness throughout cell division, will be the major topic of investigation in this work. This semester will be devoted to becoming proficient with the instruments and methods needed for the study, including the care of mice used as the model organism. Furthermore, a thorough comprehension of pertinent subjects will be created. This work aims to further knowledge of muscle regeneration and possible therapeutic uses by clarifying the molecular controls of skeletal muscle stem cell quiescence and activation. Molecular Regulation of Skeletal Muscle Stem Cell Quiescence and Activation Supervisor: CHEUNG Tom / LIFS Student: ZHOU Ruiting / BISC Course: UROP 1100, Summer During aging, the molecular regulation of stem cell quiescence and activation is believed to play a vital role in somatic regeneration. The lab focused on mice muscle stem cells and discovered the relationship between CPEB4 and muscle stem cell function previously. CPEB4, an RNA-binding protein, regulates and controls mitochondrial metabolism. Also, the inhibition of mitochondrial and CPEB4 functions is related to cell senescence (Wenshu et al., 2023). This program aims to explore more information on the function and regulation of CPEB4 on muscle stem cells. A comparison of the CPEB4 concentration between CPEB4 knockin mice and YFP mice was conducted using data analysis and imagery analysis. Molecular Regulation of Skeletal Muscle Stem Cell Quiescence and Activation Supervisor: CHEUNG Tom / LIFS Student: ZHUANG Boyi / BCB Course: UROP 1100, Summer Muscle stem cells, commonly referred to as satellite cells, are cells capable of replenishing damaged muscle tissues in cases of injuries. Satellite cells are stem cells that remain quiescent until signaled in events of muscle injuries. Under in vitro conditions, growth factors, such as in horse serum, lead to the proliferation and differentiation of the satellite cell, which can happen in various degrees in different concentrations of horse serum. Here, we determine the effect of horse serum in this dynamic regulation and whether an optimal concentration in the medium can optimize the increase in stem cell pool size.
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