School of Science Division of Life Science 19 DNA Replication-initiation Proteins in Budding Yeast Supervisor: LIANG Chun / LIFS Student: YUAN Annie / BCB Course: UROP 1100, Spring The objective of this study was to examine the inhibitory effect of CM2 on both normal and cancer cells during different phases of the cell cycle. CM2 was found to disrupt the loading of MCM proteins onto the pre-RC complex. In normal cells, a checkpoint mechanism was observed, leading to cell cycle arrest in the M/G1 phase. This resulted in better cell survival compared to cells treated in the S phase. In contrast, the lack of a checkpoint in cancer cells led to an abortive S phase and decreased cell survival. Additionally, senescence-related activity was observed in the S phase of normal cells through phase contrast photos and western blotting. DNA Replication-initiation Proteins in Human Cells Supervisor: LIANG Chun / LIFS Student: DONGRE Jayad Deepak / BCB Course: UROP 1100, Fall Mutations in the DNA sequences encoding for proteins involved in cell growth and division can result in uncontrolled cell growth to form tumours, a disease widely known as cancer. The tumours have the ability to metastasise into another location in the body and interfere with the normal functioning of body tissues, making them exceptionally life-threatening. Many anticancer drugs have been used for the treatment of cancer, including chemotherapeutic agents, but the key limitations for these has been their non-specificity to target only cancer cells; normal cells are also targeted and destroyed. This research paper focuses on the identification of a new anticancer drug named NJ4 which could possibly be used to target the pre-replication complex (pre-RC) formed during the G1 phase of the cell division cycle (CDC). Since the pre-RC is specifically put together when cells are dividing and cancerous cells are constantly multiplying, it is speculated that NJ4 will only accumulate in cancer cells and keep normal cells safe. DNA Replication-initiation Proteins in Human Cells Supervisor: LIANG Chun / LIFS Student: XIONG Yule / BIBU Course: UROP 1100, Spring The cell cycle is a complex process that involves interphase and mitosis, with checkpoints ensuring the correct replication and division of cellular components. Pre-replication complex assembly is crucial for accurate genome replication, and the development of cancer is caused by unchecked cell proliferation. Surgery, radiation therapy, chemotherapy, and targeted therapy are available cancer treatment methods, but a new drug, NJ4, is being researched for its potential to inhibit and treat cancer by targeting the pre-RC protein. The drug concentration and operation time were explored in experiments using Hela cells, with flow cytometry and cell counting confirming the drug's efficacy in blocking cell activity and preventing normal cell division. NJ4 has the potential to be an effective treatment option for cancer.
RkJQdWJsaXNoZXIy NDk5Njg=