School of Science Division of Life Science 22 Molecular Regulation of Axon Regeneration Supervisor: LIU Kai / LIFS Student: TAN Lianxing / BCB-IRE Course: UROP 4100, Fall ATX is involved in various biochemical processes, yet its role in axonal regeneration remains elusive. The extent to which the downstream receptors of ATX, LPA1-6, contribute to the repair of injured axons has not been thoroughly reported. Utilizing in vitro culture of mouse dorsal root ganglion (DRG) cell culture as a model, we previously reported that inhibition of ATX aids axonal regrowth following DRG replating. In this current study, we observed a decrease in ATX expression levels postinjury in mice, albeit with differences noted between the peripheral nervous system (PNS) and the central nervous system (CNS). In vitro studies suggest that this regenerative effect appears to be mediated through LPA2 and LPA3. Moreover, we found that a decrease in the expression level of Nsun2, an RNA methyltransferase, fosters axonal growth, potentially through the regulation of ATX expression. Molecular Regulation of Axon Regeneration Supervisor: LIU Kai / LIFS Student: WONG Chun Man / BIBU Course: UROP 1100, Spring With the aim of understanding how drugs can be developed from newly identified pathways and targets, the drug development process and important criteria for a target to be druggable are briefly discussed. Cancer therapies including target therapies and immunotherapies are discussed as a case study to understand common challenges during the development process. Novel approaches to cancer therapies are also investigated to seek inspiration in addressing diseases. All these are then put back into the subject of axon regeneration where strategies regarding the INFɣ-cGAS-STING pathway are discussed to understand potential innovations in drug development for this discovery. With that in mind, some current approaches in drug development are briefly discussed.
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