School of Science Division of Life Science 36 Genetic Studies on Stem Cell Regulation Supervisor: XIE Ting / LIFS Student: LEE Wing Yan / BIBU Course: UROP 2100, Fall Stem cells have the ability to continuously undergo self-renewal and differentiation. However, the molecular mechanisms underlying the self-renewal and differentiation of stem cells remain largely unclear. In this project, using the Gal4-UAS system, I carried out the knockdown of 14 Drosophila genes with nos-Gal4, c587Gal4, and Bab1-Gal4 in germline, differentiation niche and self-renewal niche, respectively. I have found some genes that are important for germline stem cell development. Taken together, my results have provided insights in understanding the genes that facilitate the regulation of stem cell self-renewal and differentiation, within the germarial stem cell niche. Genetic Studies on Stem Cell Regulation Supervisor: XIE Ting / LIFS Student: ONG Gavyn Kye Wen / BCB Course: UROP 2100, Fall Studying stem cell regulation is crucial for understanding tissue development and homeostasis. Genetic screening in model organisms provides valuable insights into the underlying mechanisms governing stem cell behaviour. This study focuses on investigating stem cell regulation using genetic screening techniques in Drosophila melanogaster, a well-established model organism. By employing a combination of targeted gene knockdown and overexpression strategies, we aim to identify key genes and pathways that modulate stem cell activity. Through comprehensive phenotypic analyses, we will elucidate the roles of these genes in stem cell maintenance, proliferation, and differentiation. The findings from this study will contribute to our understanding of stem cell regulation and have implications for regenerative medicine and disease modelling. Genetic Studies on Stem Cell Regulation Supervisor: XIE Ting / LIFS Student: PRINTER Roshni Olga / BIBU Course: UROP 1100, Fall The aim of this project is to conduct a genetic screening in Drosophila, to identify genes that play a role in germline stem cell (GSC) development. The genes involved in the regulation of phosphatases and kinases, which are essential for GCS development, were screened using RNAi lines. Immunostaining and confocal imaging techniques were used to identify anormal phenotypes, such as GSC loss or reduction and accumulation of CB (cytoblast) in the germarium. While further investigations are necessary to validate the impact of these genes on GSC development, this study is an initial step in exploring their significance, in order to establish confirmation of the function of genes.
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