School of Science Division of Life Science 38 Genetic Studies on Stem Cell Regulation Supervisor: XIE Ting / LIFS Student: YOON Dayeong / BCB Course: UROP 2100, Spring UROP 3200, Summer Stem cells possess regeneration and restoration of damaged tissues. It is invaluable in both medical investigations and treatments. Therefore, this investigation aimed to delve into the role of metabolic elements in stem cells that govern gene expression in germline stem cell (GSC). This research primarily focuses on germline stem cells, which eventually develop into eggs. Thus, the study concentrates on these key elements: niche cells, germline stem cells and Cytoblasts. Consequently, the Drosophila germarium is an ideal setting conducive to the growth of these elements. Within this study, 50 distinct Drosophila RNAi lines are used to modulate the activity of metabolic factors, pinpointing potential genes instrumental in regulating GSC development. Subsequently, following this experiment, ten samples are singled out for further in-depth analysis. Genetic Studies on Stem Cell Regulation Supervisor: XIE Ting / LIFS Student: ZENG Crystal Ziwei / BTGBM Course: UROP 1100, Spring Stem cells, with its ability to renew and differentiate, provides high potential in various fields, in particular, in the field of medicine for treating various diseases or for cell research. However, the genes that are required in the niche and intrinsically for controlling stem cell self-renewal, differentiation and ageing still remain unclear. The project will utilize Drosophila RNAi lines for genetic screening by using the Gal4/UAS system to knockdown genes and identify those affecting the germline stem cells. Three Gal4s, nos-Gal4, c587-Gal4, and bab1-Gal4 were used, and they express in germline, differentiation niche, and self-renewal niche respectively. In identifying these specific genes, further investigation into the regulation of stem cells may be conducted.
RkJQdWJsaXNoZXIy NDk5Njg=