School of Engineering Department of Chemical and Biological Engineering 73 Interdisciplinary Bioengineering: Microfluidic Device for Cell Manipulation/Culture and Molecular Biology Supervisor: WU Angela Ruohao / CBE Student: WU Tianyue / PHYS Course: UROP 1100, Fall This report summarizes the 2D PDMS Microfluidic device fabrication protocols and basic cell culturing skills I learnt in the UROP1100 course during 2023-24 fall semester, with a special focus on the rationales behind these practices. It covers topics from chip design principles, mold fabrication with SU-8 photolithography, to PDMS soft lithography and cell culturing, providing an overview to the whole preparation process of Microfluidic experiments with clear and detailed steps. The challenges in fabrication and various applications of Microfluidics in biological analysis and reconstructing in vivo structures are also discussed to explore the interconnection between the designs and their purposes.
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