School of Science Department of Chemistry 4 Single Molecule Manipulation and Characterization of Biomolecules Supervisor: HUANG Jinqing / CHEM Student: GUO Yichen / CHEM Course: UROP 1100, Fall UROP 2100, Spring The study of the mechanism of DNA and drug interactions by Optical Tweezer has been conducted. The Lambda DNA with 48,524 bp has no significant intercalations in mismatch probe drug solutions. An improved set up using Lambda DNA handle linked c-c base mismatched hairpin were pulled under a constant velocity for preliminary analysis of hairpin base pair opening position and it is further pulled in various concentrations of Pt centred Pt2 mismatch probe anti-cancer drug solutions. The drug and DNA interactions was tracked through the raw data of the Force distance pulling curve in drug compared to the graph in buffer. The size of sterically bulky ligands on the drug complex prohibits the insertion to bind with DNA under the applied force. Tether is confirmed to have no drug intercalation in general to the lambda handle but the opening of hairpin has been stabilized under drug solutions. The size control of ligands in drug synthesis is necessary to optimized with the separation distance between base pairs to interact in expected time or distance under zero force for better selectivity. Single Molecule Manipulation and Characterization of Biomolecules Supervisor: HUANG Jinqing / CHEM Student: XU Tongle / LIFS Course: UROP 1100, Spring UROP 2100, Summer Mitochondrial dysfunction plays a significant role in the pathogenesis of Parkinson’s Disease (PD). αsynuclein is directly linked to PD’s pathology and is distributed throughout the whole cell, including within mitochondria. As the interaction to form liquid-liquid phase separation (LLPS) between α-synuclein, TFAM and mitochondrial genome DNA (mtDNA) being evidenced, our recent work reveals the molecular feature for the in vitro system under pathologic-related condition. α-synuclein shows a close spatial pattern with TFAM on mtDNA while disrupting the preferential binding of TFAM on the 12940-19890 bp of mtDNA. Correspondingly, mtDNA genes show altered transcription level. These findings shade light on the detailed pathology for Parkinson’s Disease.
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