School of Science Division of Life Science 23 Human Complex Disease Genomics and Bioinformatics Supervisor: LIANG Chun / LIFS Co-Supervisor: XUE Hong / LIFS Student: IU Hei Ching / COSC Course: UROP 1100, Fall Meningioma, a type of primary brain tumor originating in the meninges, is the most prominent type of brain cancer to occur in humans, accounting for approximately 30% of cases. In attempt to identify correlations between oncogene sequences and the recurrence of meningioma in patients, this project utilizes cutting edge genomic and bioinformatic technologies to analyze genomic information from different individuals, specifically involving inter-Alu PCR amplification of genomic DNA of interest and the construction of a DNA library for next generation sequencing (NGS), followed by bioinformatic analysis of results. Human Complex Disease Genomics and Bioinformatics Supervisor: LIANG Chun / LIFS Co-supervisor: XUE Hong / LIFS Student: KO Ching Chi / BCB TJANDRA Claresta Audrey / BIBU Course: UROP 1100, Fall Brain cancer is closely linked to specific genetic variations, often concentrated in regions known as variant hotspots. Alu elements, a class of transposable elements, play a crucial role in the formation of these hotspots through high-frequency recombination, leading to copy number variations. In this study, we focused on sequencing regions surrounding Alu elements using Alu Polymerase chain reaction (PCR), employing primers specifically designed for targeting these sequences. Blood samples from patients with metastatic brain cancer were collected, and DNA was extracted and amplified using Inter-Alu PCR. The resulting fragments were size selected to 300-400 bp, allowing for the construction of AluScan DNA libraries. This research highlights the potential of cancer genomics in enhancing clinical treatment strategies for complex diseases. Human Complex Disease Genomics and Bioinformatics Supervisor: LIANG Chun / LIFS Co-supervisor: XUE Hong / LIFS Student: MAK Wing Yi / BIBU Course: UROP 1100, Fall Extracted from Chinese medicine, baicalein can treat a wide range of medical conditions including cancer and posttraumatic stress disorder. Therefore, a series of investigations were taken to test on the effect of different concentrations of baicalein in mice and in vitro. For testing the effective dosage of anxiolytic and anti-depression effects, animal behavioural tests were conducted, including elevated plus maze tests and tail suspension tests. 90 mg/kg Baicalein in 11% DMSO showed the most significant effect among the three testing concentrations. However, no significant anti-depression effect was observed when the same set of concentrations was used. To evaluate the anti-cancer effect of different concentrations of baicalein, cell migration and wound healing assays were performed and it was found that baicalein exhibits anti-cancer effects on HeLa cells when the concentration is above 200 μM. Thus, baicalein demonstrated its potential in treating anxiety and cancer.
RkJQdWJsaXNoZXIy NDk5Njg=