School of Science Division of Life Science 43 Genetic Studies on Stem Cell Regulation Supervisor: XIE Ting / LIFS Student: CHEN Yanyu / BCB Course: UROP 3200, Fall M6A modification has been found to control multiple biological processes, and one of the readers of m6A modification has been discovered to be essential in germline stem cell development in Drosophila ovaries. However, the detailed mechanism of how m6A modification and Ythdf regulate stem cells remains unclear. To figure out the mechanism, this study used Gal4-driving RNAi knockdown Drosophila lines to screen Ythdfbinding proteins that are involved in stem cell regulation. In this work, thirteen genes coding for Ythdfbinding proteins were found to be essential for stem cell development. The outcome facilitates further research into the intricate mechanisms and may contribute to a deeper comprehension of RNA modification in stem cell control. Genetic Studies on Stem Cell Regulation Supervisor: XIE Ting / LIFS Student: SUH Daeun / BCB Course: UROP 3200, Fall Stem cell is a unique cell type that can differentiate into specialized cells or produce another self-renewing stem cell. It is presumed that tissue aging could be caused by reduction of stem cell number and/or activity. In female Drosophila, Germline stem cells (GSCs) undergo normal aging process; GSC division rate progressively declines with age. However, overexpression of superoxide dismutase (SOD), an enzyme that helps eliminate free oxygen species, in their niche can dramatically slow down GSC aging. Our RNA-seq results suggest that expression of some genes such as Bru3, Cut, Egr, Ena, and Esg in cap cells are upregulated by SOD1 overexpression, and others like Cbs are downregulated. In this study, these five genes are knocked down quantified the number of cap cells (CCs), GSCs, and cystoblasts (CBs) at 1 week, 3 weeks, 5 weeks, 7 weeks and 9 weeks will be quantified, to see if this knock down can accelerate or slow down age-dependent GSC loss. Genetic Studies on Stem Cell Regulation Supervisor: XIE Ting / LIFS Student: YOON Dayeong / BCB Course: UROP 4100, Fall Stem cells are vital for medical research and treatment because of their critical function in tissue regeneration and repair. The purpose of this work is to examine the effects of metabolic variables on germline stem cell (GSC) gene expression. GSCs are the main focus since they develop into eggs. The study focusses on three essential elements: cytoblasts, germline stem cells, and niche cells. For these ingredients to develop, the Drosophila germarium offers the perfect habitat. Ten distinct Drosophila RNAi lines are used in this study to alter the activity of metabolic factors and find putative genes that are essential for controlling the growth of GSCs. Ten samples are then chosen for additional in-depth examination after this experimentation.
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